c-Fos Recombinant Rabbit Monoclonal Antibody [PSH07-51]

About this product

Protein c-Fos is a proto-oncogene that is the human homolog of the retroviral oncogene v-fos. It is encoded in humans by the FOS gene. It was first discovered in rat fibroblasts as the transforming gene of the FBJ MSV (Finkel–Biskis–Jinkins murine osteogenic sarcoma virus). It is a part of a bigger Fos family of transcription factors which includes c-Fos, FosB, Fra-1 and Fra-2. It has been mapped to chromosome region 14q21→q31. c-Fos encodes a 62 kDa protein, which forms heterodimer with c-jun (part of Jun family of transcription factors), resulting in the formation of AP-1 (Activator Protein-1) complex which binds DNA at AP-1 specific sites at the promoter and enhancer regions of target genes and converts extracellular signals into changes of gene expression. It plays an important role in many cellular functions and has been found to be overexpressed in a variety of cancers.

Specifications

Catalog number

HA722839

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Activator protein 1 antibody AP 1 antibody C FOS antibody Cellular oncogene c fos antibody Cellular oncogene fos antibody FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody FBJ murine osteosarcoma viral oncogene homolog antibody FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus antibody FOS antibody FOS protein antibody FOS_HUMAN antibody G0 G1 switch regulatory protein 7 antibody G0/G1 switch regulatory protein 7 antibody G0S7 antibody Oncogene FOS antibody p55 antibody proto oncogene c Fos antibody Proto oncogene protein c fos antibody Proto-oncogene c-Fos antibody v fos FBJ murine osteosarcoma viral oncogene homolog antibody

Abbreviation

FOS, G0S7

Swiss Prot

P01100 Human, P01101 Mouse, P12841 Rat

Cellular Locus

Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human c-Fos aa 221-270.

Isotype

IgG

Clone

PSH07-51

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IHC-P, IF-Cell, ChIP, WB, IHC-Fr

Positive Control

Human brain tissue, mouse brain tissue, mouse hippocampus tissue, HeLa cells serum starved for 40 hours then add 20% FBS for 2 hours, RAW264.7 cells serum starved for 16 hours then add 20% FBS for 4 hours, C6 cells serum starved for 16 hours then add 10% FBS for 30 minutes, HeLa serum starved for 40 hours then add 20% FBS for 2 hours cell lysate, RAW264.7 serum starved for 16 hours then add 200nM PMA for 4 hours cell lysate, C6 serum starved for 16 hours then add 10% FBS for 30 minutes cell lysate.

Concentration

1 mg/mL

Dilution

IHC-P: 1:200-1:1,000 ;IF-Cell: 1:100-1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;WB: 1:2,000 ;IHC-Fr: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 41 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes