Slit2 Recombinant Rabbit Monoclonal Antibody [JE31-87]

About this product

SLIT2 thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

Specifications

Catalog number

HA721407

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Drad 1 antibody E030015M03Rik antibody E130320P19Rik antibody FLJ14420 antibody OTTHUMP00000158695 antibody OTTHUMP00000217852 antibody OTTHUMP00000217853 antibody OTTHUMP00000217854 antibody Slil 3 antibody Slil3 antibody Slit 2 antibody Slit homolog 2 (Drosophila) antibody Slit homolog 2 antibody Slit homolog 2 protein antibody Slit homolog 2 protein C-product antibody Slit-2 antibody Slit2 antibody SLIT2_HUMAN antibody

Abbreviation

SLIT2, SLIL3

Swiss Prot

O94813 Human, Q9R1B9 Mouse, Q9WVC1 Rat

Cellular Locus

Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Slit2 aa 1,380-1,529 / 1,529.

Isotype

IgG

Clone

JE31-87

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, FC

Positive Control

HEK-293 cell lysate, 293T cell lysate, PC-3M cell lysate, Raji cell lysate, HCT 116 cell lysate, HeLa cell lysate, rat pancreas tissue lysate, SH-SY5Y cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, A549.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:50-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 170 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes