Recombinant Human INHBA Protein (Sumo Tag)

Specifications

Catalog number

PDEH101131-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. The activin and inhibin protein complexes are both dimeric in structure, and, in each complex, the two monomers are linked to one another by a single disulfide bond. Activin is composed of two β subunits, βA βA (activin A), βB βB (activin B), or βA βB (activin AB) . Inhibin is composed of an alpha and one of two β subunits, βA (inhibin A) or βB (inhibin B) . Activins are produced in many cell types and organs, such as gonads, pituitary gland, and placenta. In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization. It participates in androgen synthesis enhancing LH action in the ovary and testis. In the male, activin enhances spermatogenesis. Also, Activin plays a role in wound repair and skin morphogenesis. Activin is strongly expressed in wounded skin, and overexpression of activin in the epidermis of transgenic mice improves wound healing and enhances scar formation. Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and kidney. There is also evidence showed that lack of activin during development results in neural developmental defects.

Abbreviation

INHBA

Synonyms

Activin A; INHBA; Inhibin beta A chain

UniProt

P08476

Accession Number

P08476

Expression System

E.coli

Tag

N-Sumo

Sequence

Gly311-Ser426

Field of Research

Developmental Biology; Cancer; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.6 kDa

Observed Molecular Weight

33 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human