Recombinant Human S100A8 Protein (GST Tag)

Specifications

Catalog number

PDEH100651-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

S100A8/S100A9 Heterodimer; S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70) . There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Abbreviation

S100A8

Synonyms

Protein S100-A; S100a; Kcnip; KCHIP; MRP; S100A8;60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8; Calgranulin A; Calgranulin-A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MRP-8; Protein S100-A8; CALP; KCNIP4; KCHIP4; AI323541; B8Ag; BEE11; Calprotectin; Calprotectin L1L subunit; Chemotactic cytokine CP-10; included; Migration inhibitory factor-related protein 8; Myeloid-related protein 8; Neutrophil cytosolic 7 kDa protein; Pro-inflammatory S100 cytokine; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A) ; S100 calcium-binding protein A8; S100A8/S100A9 complex; S10A8; Urinary stone protein band A

UniProt

P05109

Accession Number

P05109

Expression System

E.coli

Tag

N-GST

Sequence

Met1-Glu93

Field of Research

Neuroscience; Cancer; Immunology; Signal Transduction; Cell Biology; Epigenetics and Nuclear Signaling

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.2 kDa

Observed Molecular Weight

40 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human