p53 (acetyl K382) Recombinant Rabbit Monoclonal Antibody [PSH06-87]

About this product

p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Specifications

Catalog number

HA722762

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody FLJ92943 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody tumor antigen p55 antibody Tumor protein 53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody

Abbreviation

TP53, P53

Swiss Prot

P04637 Human, P02340 Mouse

Cellular Locus

Cytoplasm, Nucleus, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within human p53 (acetyl K382) aa 344-393.

Isotype

IgG

Clone

PSH06-87

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell

Positive Control

HepG2 starved overnight treated with 30μg/mL etoposide for 7 hours then add 500ng/mL TSA for 3 hours cell lysate, NIH/3T3 treated with 400nM TSA and 0.5μM Doxorubicin for 24 hours cell lysate, NIH/3T3 cells treated with 400nM TSA and 0.5μM Doxorubicin for 24 hours.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes