EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL — image 1 of 2

Product details

EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL

Catalog number BNC041176-500

Price for BNC041176-500

Size / count 1x 500 µL

About this product

This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1) . It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC041176-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Early Mitotic Inhibitor-1 (EMI1) ; FBX5; Fbxo31
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: FBXO5
Gene ID: 26271
NCBI Gene ID: 520506
UniProt: Q9UKT4
Swiss Prot: Q9UKT4
Host: Mouse
Species Reactivity: Human
Reactivity: EMI1
Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
Target Antigen: EMI1
Clonality: Monoclonal
Clone: EMI1/1176
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Field of Research: Cell cycle
Positive Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 56 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF405S

Frequently Asked Questions

What is the catalog number for EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL?

The catalog number for EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL is BNC041176-500.

What sizes are available for EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL?

EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL in stock?

EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL?

EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL?

Estimated delivery time for EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL?

The safety data sheet for EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02b0061270361e109697/msds/bnc041176-500.pdf.

What is EMI1 (EMI1/1176), CF405S conjugate, 0.1mg/mL used for?

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.