Histone H1 (AE-4), CF740 conjugate, 0.1mg/mL

About this product

Eukaryotic histones are basic, water-soluble nuclear proteins that form hetero-octameric nucleosome particles. They wrap 146 base pairs of DNA in a left-handed supealpha-helicalturn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC740096-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

H1 (0) |H1.1|H1.2|H1.3|H1.4|H1.5|H1A|H1F0|H1F1|H1F2|H1F3|H1F4|H1F5|H1FNT|H1FOO|H1FT|H1FV|H1FX|H1t|H1T2|H1X|HANP1|His1|HisC|HIST1|HIST1H1A|HIST1H1B|HIST1H1C|HIST1H1D|HIST1H1E|HIST1H1T|Oocyte-specific histone H1|Testicular H1 histone

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

H1

Gene ID

3005

NCBI Gene ID

226117 & 97358

UniProt

Multiple

Swiss Prot

Multiple

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Reactivity

Histone H1

Immunogen

Nuclei of human leukemia biopsy cells

Target Antigen

Histone H1

Clonality

Monoclonal

Clone

AE-4

Type

Primary

Source

Animal

Origin

Animal

Applications

CHiP (published) |Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |IP (published) |WB (published)

Field of Research

Organelle markers

Positive Control

HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

~30 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Psychiatry Neurosci (2009) 34(3): 232-237. (Western) Genes & Dev (2011) 25:845-862. (CHiP)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), Bos taurus (bovine)

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF740