• Certified Lab Equipment & Reagents
  • Fast Dispatch on In-Stock Items
  • Expert Scientific Support

Product details

CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL

Catalog number BNC740177-100

Price for BNC740177-100

Sign In for Pricing

Size / count 1x 100 µL

About this product

Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V) . Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC740177-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
ICAMR; Intercellular adhesion molecule 3 (ICAM3)
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
ICAM3
Gene ID
3385
NCBI Gene ID
354563
UniProt
P32942
Swiss Prot
P32942
Host
Mouse
Species Reactivity
Human
Reactivity
CD50
Immunogen
Stimulated human leukocytes
Target Antigen
CD50
Clonality
Monoclonal
Clone
101-1D2
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, surface (published) |Functional studies (published) |ELISA (published)
Field of Research
Immunology
Positive Control
HL-60 or THP-1 cells. Lymph node and tonsil
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% rBSA, 0.05% azide
Molecular Weight
110-160 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp, W. et. al. Leucocyte Typing IV, p541, 667-670, 1087, Oxford Univ. Press, 1989. Schlossman SF, et. al. Leucocyte Typing V, p1542-1547, 2011, Oxford Univ. Press, 1993. Eur J Immunol (1993) 23: 1508-1512. (IP, Flow) Eur J Immunol (1994) 24: 1377-1382. (functional studies) J of Immunol (1995) 154(6):3015-24. (ELISA)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Mus musculus (mouse), Bos taurus (bovine)
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF740

Frequently Asked Questions

What is the catalog number for CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL?

The catalog number for CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL is BNC740177-100.

What sizes are available for CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL?

CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL in stock?

CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL?

CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL?

Estimated delivery time for CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL?

The safety data sheet for CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02a5061270361e10584b/msds/bnc740177-100.pdf.

What is CD50 (ICAM-3) (101-1D2), CF740 conjugate, 0.1mg/mL used for?

Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V) . Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signal

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

You Might Also Like
CD44 Standard (DF1485), CF568 conjugate, 0.1mg/mL
CD44 Standard (DF1485), CF568 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC681037-100
Insulin (2D11-H5), CF640R conjugate, 0.1mg/mL
Insulin (2D11-H5), CF640R conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC400030-500
TNF alpha (J2D10), CF640R conjugate, 0.1mg/mL
TNF alpha (J2D10), CF640R conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC400994-100
Von Willebrand Factor (3E2D10), CF740 conjugate, 0.1mg/mL
Von Willebrand Factor (3E2D10), CF740 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC740633-500
MUC18 / Mucin 18 / CD146 (OJ79c), CF640R conjugate, 0.1mg/mL
MUC18 / Mucin 18 / CD146 (OJ79c), CF640R conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC400676-500
CD3e (RIV9), CF568 conjugate, 0.1mg/mL
CD3e (RIV9), CF568 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC680322-100
CD5 (Cris-1), CF647 conjugate, 0.1mg/mL
CD5 (Cris-1), CF647 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC470176-100
CD100 (Semaphorin-4D) (A8), CF405S conjugate, 0.1mg/mL
CD100 (Semaphorin-4D) (A8), CF405S conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC040218-500