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Product details

Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL

Catalog number BNC740488-100

Price for BNC740488-100

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Size / count 1x 100 µL

About this product

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC740488-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
TOP1MT
Gene ID
116447
NCBI Gene ID
528574
UniProt
Q969P6
Swiss Prot
Q969P6
Host
Mouse
Species Reactivity
Human
Reactivity
Topoisomerase I, Mitochondrial
Immunogen
Recombinant full-length human TOP1MT protein
Target Antigen
Topoisomerase I, Mitochondrial
Clonality
Monoclonal
Clone
TOP1MT/488
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Field of Research
Cell cycle
Positive Control
A431 cells. Heart, Skeletal muscle, brain or fetal liver.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% rBSA, 0.05% azide
Molecular Weight
70 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2b, kappa
Species
Mus musculus (mouse), Bos taurus (bovine)
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF740

Frequently Asked Questions

What is the catalog number for Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL?

The catalog number for Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL is BNC740488-100.

What sizes are available for Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL?

Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL in stock?

Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL?

Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL?

Estimated delivery time for Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL?

The safety data sheet for Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02b5061270361e10b17b/msds/bnc740488-100.pdf.

What is Topoisomerase I, MT (TOP1MT/488), CF740 conjugate, 0.1mg/mL used for?

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous antican

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

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