ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL

Catalog number BNC740811-100

Price for BNC740811-100

Size / count 1x 100 µL

About this product

ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC740811-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; Zeta chain associated protein kinase 70kDa
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: ZAP70
Gene ID: 7535
NCBI Gene ID: 234569
UniProt: P43403
Swiss Prot: P43403
Host: Mouse
Species Reactivity: Human
Reactivity: ZAP70
Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
Target Antigen: ZAP70
Clonality: Monoclonal
Clone: 2F3.2
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified)
Field of Research: Immunology
Positive Control: Jurkat cells. Tonsil or lymph node.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% rBSA, 0.05% azide
Molecular Weight: 70 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), Bos taurus (bovine)
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF740

Frequently Asked Questions

What is the catalog number for ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL?

The catalog number for ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL is BNC740811-100.

What sizes are available for ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL?

ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL in stock?

ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL?

ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL?

Estimated delivery time for ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL?

The safety data sheet for ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029f061270361e10345a/msds/bnc740811-100.pdf.

What is ZAP70 (2F3.2), CF740 conjugate, 0.1mg/mL used for?

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.