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Product details

FNC Coating Mix®, 100 ml

Catalog number 0407

Price for 0407

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Size / count 100 mL

About this product

<font size="3">FNC Coating Mix® is a specially formulated serum-free tissue culture reagent containing fibronectin collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated. Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaES® offers several different serum-free media that when used in conjunction with FNC Coating Mix® increase the growth of cell cultures dramatically.</font><div><font size="3"><br></font></div><div><font size="5" color="#133094"><b style="">Key Features </b></font><font size="3"> </font><ul style="font-size: medium;"><li><font size="3"><font color="#0015ab"><b>Enhanced Cell Attachment →</b></font> Improves attachment of adherent mammalian cells to plastic culture surfaces. </font></li><li><span style="font-size: medium;"><b><font color="#0015ab">Serum-Free Coating Reagent →</font></b> Designed for cell culture workflows using appropriate serum-free culture media.</span></li><li><span style="font-size: medium;"><b><font color="#0015ab">Extracellular Matrix Components →</font></b> Contains fibronectin, collagen and albumin to provide a cell-compatible coated surface.</span></li><li><span style="font-size: medium;"><b><font color="#0015ab">Ready-to-Use Formulation →</font></b> Supplied as a liquid coating reagent for convenient preparation of culture surfaces.</span></li><li><span style="font-size: medium;"><b><font color="#0015ab">Supports Monolayer Formation → </font></b>Promotes efficient cell attachment and monolayer development in suitable adherent cell culture systems.</span></li><li><span style="font-size: medium;"><b><font color="#0015ab">Suitable for Fastidious Cell Types →</font></b> Particularly useful for adherent cells that require enhanced substrate attachment, including certain epithelial cell cultures.</span></li></ul><div style="font-size: medium;"><br></div><div style=""><font size="5" color="#133094"><b style="">Typical Coating Workflow </b></font><font size="3"> For compatible cell culture applications : <b>1. Coat →</b> Cover the culture surface completely with FNC Coating Mix®. <b>2. Incubate →</b> Allow the coating to contact the surface according to the applicable protocol. <b>3. Remove Excess →</b> Aspirate the remaining coating solution. <b>4. Seed Cells → </b>Add the prepared cell suspension to the coated culture surface. <br></font></div><div style=""><font size="3">The manufacturer's associated product information describes a coating volume of approximately 0.2 mL/cm², with the coating typically incubated for about 30 seconds before removal.</font></div></div>

Specifications

Catalog number
0407
Size / count
100 mL
Dry ice
No
Hazardous
No
Discontinued
No
Trademark Notice
Registered Trademark
HS Code
3821000000
Applications
<font size="5" color="#133094"><b>FNC Coating Mix® Applications </b></font> <ul><li><font size="3"><font color="#0015ab"><b>Adherent Cell Culture →</b></font> Preparation of coated plastic surfaces to improve attachment of adherent mammalian cells.</font></li><li><font size="3"><b><font color="#0015ab">Epithelial Cell Culture →</font></b> Supports attachment and growth of epithelial cell cultures, including difficult-to-attach cell types.</font></li><li><font size="3"><b><font color="#0015ab">Primary Cell Culture →</font></b> Used as a substrate coating in appropriate primary adherent cell culture workflows.</font></li><li><font size="3"><b><font color="#0015ab">Cell Monolayer Formation →</font></b> Supports efficient attachment and establishment of adherent cell monolayers.</font></li><li><font size="3"><b><font color="#0015ab">Cell-Based Research →</font></b> Suitable for cell culture, cell biology and experimental tissue culture workflows. FNC Coating Mix® has also been used in published research involving human corneal endothelial cells, renal epithelial cells and other adherent cell models.</font></li></ul>
Shipping Conditions
Cryopacks
Storage Conditions
4°C
Shelf Life
2 Years
Related Products
0401, 0402, 0403, 0404, 0407, 0407H, 0415
FAQ
<font size="3"><b><font color="#0015ab">What is FNC Coating Mix®? </font></b><span style="color: rgb(0, 19, 171); font-weight: 700;">→ </span>FNC Coating Mix® is a serum-free cell attachment reagent used to coat plastic culture surfaces and enhance attachment of adherent mammalian cells. <b><font color="#0015ab">What does FNC Coating Mix® contain? </font></b><span style="color: rgb(0, 19, 171); font-weight: 700;">→ </span>FNC Coating Mix® contains fibronectin, collagen and albumin, which contribute to its extracellular-matrix-like coating properties. <b><font color="#0015ab">What is FNC Coating Mix® used for? </font></b><span style="color: rgb(0, 19, 171); font-weight: 700;">→ </span>It is used to improve cell attachment, spreading and monolayer formation in adherent cell culture systems. <b><font color="#0015ab">Can FNC Coating Mix® be used for epithelial cells? </font></b><span style="color: rgb(0, 19, 171); font-weight: 700;">→ </span>Yes. It is particularly useful for certain epithelial cell cultures and other adherent cells that require improved attachment to culture surfaces. <b><font color="#0015ab">Is FNC Coating Mix® ready to use? </font></b><span style="color: rgb(0, 19, 171); font-weight: 700;">→ </span>Yes. The product is supplied as a ready-to-use liquid coating reagent. <b><font color="#0015ab">What culture surfaces can be coated with FNC Coating Mix®? </font></b><span style="color: rgb(0, 19, 171); font-weight: 700;">→ </span>FNC Coating Mix® is designed for coating plastic surfaces such as cell culture flasks, dishes and microplates.</font>

Frequently Asked Questions

What is the catalog number for FNC Coating Mix®, 100 ml?

The catalog number for FNC Coating Mix®, 100 ml is 0407.

What sizes are available for FNC Coating Mix®, 100 ml?

FNC Coating Mix®, 100 ml is available in the following size(s): 100 mL.

Is FNC Coating Mix®, 100 ml in stock?

FNC Coating Mix®, 100 ml is currently made to order / not held in immediate stock. Contact us for lead time.

What is FNC Coating Mix®, 100 ml used for?

FNC Coating Mix® is a specially formulated serum-free tissue culture reagent containing fibronectin collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

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