Phospho-Cdk1/2/3 (T14) Recombinant Rabbit Monoclonal Antibody [JE04-37]

About this product

Cyclin-dependent kinases (CDKs) are a predominant group of serine/threonine protein kinases involved in the regulation of the cell cycle and its progression, ensuring the integrity and functionality of cellular machinery. These regulatory enzymes play a crucial role in the regulation of eukaryotic cell cycle and transcription, as well as DNA repair, metabolism, and epigenetic regulation, in response to several extracellular and intracellular signals. They are present in all known eukaryotes, and their regulatory function in the cell cycle has been evolutionarily conserved. The catalytic activities of CDKs are regulated by interactions with CDK inhibitors (CKIs) and regulatory subunits known as cyclins. Cyclins have no enzymatic activity themselves, but they become active once they bind to CDKs. Without cyclin, CDK is less active than in the cyclin-CDK heterodimer complex. CDKs phosphorylate proteins on serine (S) or threonine (T) residues. The specificity of CDKs for their substrates is defined by the S/T-P-X-K/R sequence, where S/T is the phosphorylation site, P is proline, X is any amino acid, and the sequence ends with lysine (K) or arginine (R). This motif ensures CDKs accurately target and modify proteins, crucial for regulating cell cycle and other functions. Deregulation of the CDK activity is linked to various pathologies, including cancer, neurodegenerative diseases, and stroke.

Specifications

Catalog number

HA722272

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cdc 2 antibody Cdc2 antibody CDC28A antibody CDK 1 antibody CDK1 antibody CDK1_HUMAN antibody CDKN1 antibody CELL CYCLE CONTROLLER CDC2 antibody Cell division control protein 2 antibody Cell division control protein 2 homolog antibody Cell division cycle 2 G1 to S and G2 to M antibody Cell division protein kinase 1 antibody Cell Divsion Cycle 2 Protein antibody Cyclin Dependent Kinase 1 antibody Cyclin-dependent kinase 1 antibody DKFZp686L20222 antibody MGC111195 antibody p34 Cdk1 antibody p34 protein kinase antibody P34CDC2 antibody Cdc2 related protein kinase antibody cdc2-related protein kinase antibody CDC28 antibody CDC2A antibody Cdk 2 antibody CDK2 antibody CDK2_HUMAN antibody CDKN2 antibody Cell devision kinase 2 antibody Cell division protein kinase 2 antibody Cyclin dependent kinase 2 antibody cyclin dependent kinase 2-alpha antibody Cyclin-dependent kinase 2 antibody kinase Cdc2 antibody MPF antibody p33 protein kinase antibody p33 (CDK2) antibody Cdk 3 antibody Cdk3 antibody CDK3_HUMAN antibody CDKN3 antibody Cell division kinase 3 antibody Cell division protein kinase 3 antibody Cyclin dependent kinase 3 antibody Cyclin-dependent kinase 3 antibody OTTHUMP00000206828 antibody p36 antibody

Abbreviation

CDK1, CDC2, CDC28A, CDKN1, P34CDC2, CDK2, CDKN2, CDK3, CDKN3

Swiss Prot

P06493 Human, P24941 Human, Q00526 Human, P11440 Mouse, P97377 Mouse, Q80YP0 Mouse, P39951 Rat, Q63699 Rat

Cellular Locus

Nucleus, Cytoplasm, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1 protein (P06493).

Isotype

IgG

Clone

JE04-37

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

SiHa cell lysate, Caco-2 cell lysate, HeLa cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, human colon tissue, human liver tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 34 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes