Recombinant Mouse NT-ProBNP Protein (Trx Tag)

Specifications

Catalog number

PDEM100205-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

Abbreviation

NT-ProBNP

Synonyms

B-Type Natriuretic Peptide; GC-B; Natriuretic Peptide Precursor B; NT-Pro-BNP; Nppb

UniProt

P40753

Accession Number

P40753

Expression System

E.coli

Tag

N-Trx

Sequence

Ser26-Arg76

Field of Research

Cardiovascular; Signal Transduction; Tags and Cell Markers; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.6 kDa

Observed Molecular Weight

22 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse