QuicKey Pro Human HCG (Chorionic Gonadotropin) ELISA Kit

Specifications

Catalog number

E-OSEL-H0026-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

HCG

UniProt

P01215 (CGA) P0DN86 (CGβ)

Cross Reactivity

This kit recognizes HCG in samples.No significant cross-reactivity or interference between HCG and analogues was observed.

Target

HCG

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental Biology; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HCG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human HCG are added to the micro ELISA plate wells. Human HCG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human HCG.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HCG. The concentration of Human HCG in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 mIU/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 mIU/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human HCG concentrations in serum, plasma and other biological fluids.