E-Cadherin Polyclonal Antibody

Specifications

Catalog number

AN004540L-01

Size / count

25 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin) . This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

Abbreviation

E-Cadherin

UniProt

P12830

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human E-Cadherin protein expressed by E.coli

Target

Arc 1; Arc 1; CADH1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 324; CD324; CD324 antigen; cdh1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; Arc-1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CD 324; CD324 antigen; E-Cad/CTF3; epithelial calcium dependant adhesion protein; Liver cell adhesion molecule; CDH; Arc; CADH; CD antigen CD; Cleaved into: E-Cad/CTF; Cadherin; CAM 120/; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; CDH1; E-Cadherin; CADH1; Cadherin-1; Uvomorulin; E-Cad; CTF2; CAM 120/80; Epithelial cadherin; CD antigen CD324; Cleaved into: E-Cad/CTF1; CTF3; CDH1

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB

Field of Research

Signal Transduction; Cancer; Developmental Biology; Tags & Cell Markers

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

WB 1:250-1:500

Buffer

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

98 kDa

Observed Molecular Weight

120 kDa

Isotype

IgG