Recombinant Human DDIT3 Protein (GST Tag)

Specifications

Catalog number

PDEH100664-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CHOP/GADD153, also known as DNA-damage-inducible transcript 3 (DDIT3), is a basic domain-leucine zipper (bZIP) transcription factor of C/EBP family. This protein has been shown to be up-regulated by several stresses, such as amino acid or glucose starvation, endoplasmic reticulum (ER) stress, osmotic stress and hypoxia. GADD153 protein may play a role in ER stress-mediated apoptosis and in disease including diabetes, brain ischemia and neurodegenerative disease. Recombinant GADD153 fused with His-tag, was expressed in E.coli and purified by conventional chromatography techniques.

Abbreviation

DDIT3

Synonyms

DDIT; GADD; DNA damage inducible transcript; DDIT3; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; C/EBPzeta; DDIT3 / CHOP; DNA damage inducible transcript 3; CEBPZ; CHOP; CHOP10; CHOP-10; DNA damage inducible transcript 3; GADD153; C/EBP homologous protein; C/EBP Homology Protein; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer binding protein homologous protein; CHOP 10; DDIT 3; DDIT-3; DNA damage-inducible transcript 3 protein; GADD 153; Growth Arrest and DNA Damage Inducible Protein 153; Growth arrest and DNA damage inducible protein GADD153; Growth arrest and DNA damage-inducible protein GADD153; MGC4154

UniProt

P35638

Accession Number

P35638

Expression System

E.coli

Tag

N-GST

Sequence

Met1-Ala169

Field of Research

Epigenetics and Nuclear Signaling; Stem Cells; Developmental Biology; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.6 kDa

Observed Molecular Weight

43 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human