SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified)

Catalog number SPD-C5258-1mg

Discontinued

Price for SPD-C5258-1mg

Size / count 1 mg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 S protein RBD (K417T,E484K,N501Y), Fc Tag (SPD-C5258) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417T, E484K, N501Y)). The K417T, E484K, N501Y mutations were identified in the SARS-CoV-2 Gamma variant (Pango lineage: P.1; other names: 20J/501Y.V3).

Specifications

Catalog number: SPD-C5258-1mg
Size / count: 1 mg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Unconjugated
Tag: C-Fc
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (Cat. No. SPD-C5258) at 2 μg/mL (100 μL/well) can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with a linear range of 39-156 ng/mL (QC tested).
Format: Powder
Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
Additives: Trehalose
Molecular Weight: 51.1 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified)?

The catalog number for SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified) is SPD-C5258-1mg.

Is SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified) in stock?

SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified)?

SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified)?

Estimated delivery time for SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), Fc Tag (MALS verified) used for?

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.