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Product details

VD (Vitamin D) ELISA Kit

Catalog number E-EL-0012-01

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Price for E-EL-0012-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-0012-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
VD
Cross Reactivity
This kit recognizes Universal VD in samples.No significant cross-reactivity or interference between Universal VD and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Metabolism
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal VD. During the reaction, Universal VD in the sample or standard competes with a fixed amount of Universal VD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VD in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
6.25-400 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
3.75 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0147651321008423
Citation 02
https://www.sciencedirect.com/science/article/pii/S0955286322002169
Citation 03
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8553260/
Citation 04
https://europepmc.org/article/med/33295720?javascript_support=no
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of VD concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for VD (Vitamin D) ELISA Kit?

The catalog number for VD (Vitamin D) ELISA Kit is E-EL-0012.

What sizes are available for VD (Vitamin D) ELISA Kit?

VD (Vitamin D) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does VD (Vitamin D) ELISA Kit come in?

VD (Vitamin D) ELISA Kit is available in 3 different pack sizes.

Is VD (Vitamin D) ELISA Kit in stock?

VD (Vitamin D) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures VD (Vitamin D) ELISA Kit?

VD (Vitamin D) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for VD (Vitamin D) ELISA Kit?

Estimated delivery time for VD (Vitamin D) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for VD (Vitamin D) ELISA Kit?

The safety data sheet for VD (Vitamin D) ELISA Kit is available here: https://789.bio/eb/H8OKqT.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

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