VD3 (Vitamin D3) ELISA Kit

Specifications

Catalog number

E-EL-0014-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

VD3

Cross Reactivity

This kit recognizes Universal VD3 in samples.No significant cross-reactivity or interference between Universal VD3 and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal VD3. During the reaction, Universal VD3 in the sample or standard competes with a fixed amount of Universal VD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1007/s00223-022-00975-z

Citation 02

https://link.springer.com/article/10.1007/s00774-021-01303-5

Citation 03

https://www.scielo.br/scielo.php?pid=S2359-39972019005008105&script=sci_arttext

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208565/

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of VD3 concentrations in serum, plasma and other biological fluids.