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Product details

25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit

Catalog number E-EL-0015-01

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Price for E-EL-0015-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-0015-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
25-HVD3
Cross Reactivity
This kit recognizes Universal 25-HVD3 in samples.No significant cross-reactivity or interference between Universal 25-HVD3 and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Metabolism; Developmental biology
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 25-HVD3. During the reaction, Universal 25-HVD3 in the sample or standard competes with a fixed amount of Universal 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
3.13-200 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
1.88 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0006295222003781
Citation 02
https://link.springer.com/article/10.1007/s00223-022-00975-z
Citation 03
https://pubs.acs.org/doi/abs/10.1021/acsapm.9b00179
Citation 04
https://www.sciencedirect.com/science/article/pii/S0753332220306417
Citation 05
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039685/
Citation 06
https://www.frontiersin.org/articles/10.3389/fnins.2018.00141/full?report=reader
Citation 07
https://www.sciencedirect.com/science/article/pii/S0304394022002865
Citation 08
https://www.sciencedirect.com/science/article/pii/S0271531718314490
Citation 09
https://www.degruyter.com/document/doi/10.1515/jpem-2018-0554/html
Citation 10
https://www.scielo.br/scielo.php?pid=S0004-282X2019001200848&script=sci_arttext
Citation 11
https://journals.sagepub.com/doi/abs/10.1177/0300060519896892
Citation 12
https://www.degruyter.com/document/doi/10.1515/tjb-2020-0305/html
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of 25-HVD3 concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit?

The catalog number for 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit is E-EL-0015.

What sizes are available for 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit?

25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit come in?

25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit is available in 3 different pack sizes.

Is 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit in stock?

25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit?

25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit?

Estimated delivery time for 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit?

The safety data sheet for 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit is available here: https://789.bio/eb/fnrDqD.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

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