8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

Specifications

Catalog number

E-EL-0028-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

8-OHdG

Cross Reactivity

This kit recognizes Universal 8-OHdG in samples.No significant cross-reactivity or interference between Universal 8-OHdG and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Metabolism; Cardiovascular; Immunology; Cancer

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 8-OHdG. During the reaction, Universal 8-OHdG in the sample or standard competes with a fixed amount of Universal 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 8-OHdG in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41586-020-2394-6

Citation 02

https://www.sciencedirect.com/science/article/pii/S2213231721000203

Citation 03

https://www.nature.com/articles/s41419-022-05120-0

Citation 04

https://www.liebertpub.com/doi/abs/10.1089/ars.2020.8159

Citation 05

https://www.sciencedirect.com/science/article/pii/S0147651322008405

Citation 06

https://www.liebertpub.com/doi/abs/10.1089/ars.2022.0008

Citation 07

https://www.sciencedirect.com/science/article/pii/S0891584922002295

Citation 08

https://www.mdpi.com/1856270

Citation 09

https://www.mdpi.com/article/10.3390/antiox11112264

Citation 10

https://www.mdpi.com/2076-3921/11/7/1301

Citation 11

https://www.sciencedirect.com/science/article/pii/S1043661822000688

Citation 12

https://www.frontiersin.org/articles/10.3389/fimmu.2022.880578/full

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of 8-OHdG concentrations in serum, plasma and other biological fluids.