ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit

Specifications

Catalog number

E-EL-0033-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ST;5-HT

Cross Reactivity

This kit recognizes Universal ST/5-HT in samples.No significant cross-reactivity or interference between Universal ST/5-HT and analogues was observed

Target

5HT; Enteramine; Thrombocytin; Thrombotonin;3- (β-Aminoethyl) -5-hydroxyindole

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ST/5-HT. During the reaction, Universal ST/5-HT in the sample or standard competes with a fixed amount of Universal ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ST/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ST/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0016508517302718

Citation 02

https://aacrjournals.org/cancerimmunolres/article-abstract/9/9/1008/666146

Citation 03

https://pubmed.ncbi.nlm.nih.gov/34964929/

Citation 04

https://www.sciencedirect.com/science/article/pii/S0269749121013968

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555466/

Citation 06

https://www.sciencedirect.com/science/article/pii/S1043661822000354

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332222008265

Citation 08

https://www.sciencedirect.com/science/article/pii/S0753332222015037

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202200481

Citation 10

https://www.mdpi.com/2072-6643/14/19/4083

Citation 11

https://www.frontiersin.org/articles/10.3389/fcimb.2022.945851/full?utm_source=dlvr.it&utm_medium=twitter

Citation 12

https://www.sciencedirect.com/science/article/pii/S0753332219327489

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of ST/5-HT concentrations in serum, plasma and other biological fluids.