ADMA (Asymmetrical Dimethylarginine) ELISA Kit

Specifications

Catalog number

E-EL-0042-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ADMA

Cross Reactivity

This kit recognizes Universal ADMA in samples.No significant cross-reactivity or interference between Universal ADMA and analogues was observed

Target

N; N-Dimethylarginine; NG-Dimethylarginine dihydrochloride;2-Amino-5-[ (amino-dimethylaminomethylene) amino]pentanoic acid

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction; Cardiovascular

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ADMA. During the reaction, Universal ADMA in the sample or standard competes with a fixed amount of Universal ADMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ADMA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ADMA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1007/s11357-021-00507-x

Citation 02

https://www.mdpi.com/2076-3921/10/5/722

Citation 03

https://europepmc.org/article/med/35991671?javascript_support=no

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.15278

Citation 05

https://www.mdpi.com/965270

Citation 06

https://www.sciencedirect.com/science/article/pii/S1089860317302355

Citation 07

https://www.mdpi.com/937888

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1002/dmrr.2926

Citation 09

https://www.sciencedirect.com/science/article/pii/S0014299919307381

Citation 10

https://www.sciencedirect.com/science/article/pii/S1089860319301533

Citation 11

https://www.sciencedirect.com/science/article/pii/S1871403X21000910

Citation 12

https://www.scielo.br/scielo.php?pid=S0102-76382020005006205&script=sci_arttext

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of ADMA concentrations in serum, plasma and other biological fluids.