EPI (Epinephrine/Adrenaline) ELISA Kit

Specifications

Catalog number

E-EL-0045-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

EPI

Cross Reactivity

This kit recognizes Universal EPI in samples.No significant cross-reactivity or interference between Universal EPI and analogues was observed

Target

Epinephrine; adrenaline; adrenalin;4;5-β-trihydroxy-N-methylphenethylamine

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell Biology; Metabolism; Epigenetics and Nuclear Signal; Signal transduction; Neuroscience; Cardiovascular; Cancer; Biochemicals

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal EPI. During the reaction, Universal EPI in the sample or standard competes with a fixed amount of Universal EPI on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal EPI. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal EPI in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0092867422015379

Citation 02

https://www.nature.com/articles/s41423-022-00967-x

Citation 03

https://www.sciencedirect.com/science/article/pii/S0269749122010363

Citation 04

https://www.nature.com/articles/s42003-022-03694-1

Citation 05

https://www.jneurosci.org/content/early/2022/08/25/JNEUROSCI.2509-21.2022.abstract

Citation 06

https://www.sciencedirect.com/science/article/pii/S2212877819306192

Citation 07

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 08

https://link.springer.com/article/10.1186/s12970-020-00375-4

Citation 09

https://www.sciencedirect.com/science/article/pii/S0003986122002740

Citation 10

https://pubs.acs.org/doi/abs/10.1021/acsomega.2c03619

Citation 11

https://link.springer.com/article/10.1007/s00592-022-01859-2

Citation 12

https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-023-00998-z

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of EPI concentrations in serum, plasma and other biological fluids.