NA/NE (Noradrenaline/Norepinephrine) ELISA Kit

Specifications

Catalog number

E-EL-0047-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

NA; NE

Cross Reactivity

This kit recognizes Universal NA/NE in samples.No significant cross-reactivity or interference between Universal NA/NE and analogues was observed

Target

Norepinephrine; NE; noradrenaline; NA; NAd; norad;4;5-β-trihydroxyphenethylamine

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience; Cardiovascular

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal NA/NE. During the reaction, Universal NA/NE in the sample or standard competes with a fixed amount of Universal NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal NA/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal NA/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0092867422015379

Citation 02

https://www.nature.com/articles/s41423-022-00967-x

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202100808

Citation 04

https://www.sciencedirect.com/science/article/pii/S0026049522001706

Citation 05

https://link.springer.com/article/10.1007/s00395-022-00968-x

Citation 06

https://www.sciencedirect.com/science/article/pii/S0269749122010363

Citation 07

https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02489-9

Citation 08

https://link.springer.com/article/10.1186/s13046-020-01568-0

Citation 09

https://www.nature.com/articles/s42003-022-03694-1

Citation 10

https://www.jneurosci.org/content/early/2022/08/25/JNEUROSCI.2509-21.2022.abstract

Citation 11

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 12

https://www.mdpi.com/2013432

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of NA/NE concentrations in serum, plasma and other biological fluids.