6-keto-PGF1a (6-keto-prostaglandin F1a) ELISA Kit

Specifications

Catalog number

E-EL-0054-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

6-keto-PGF1a

Cross Reactivity

This kit recognizes Universal 6-keto-PGF1a in samples.No significant cross-reactivity or interference between Universal 6-keto-PGF1a and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cardiovascular

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 6-keto-PGF1a. During the reaction, Universal 6-keto-PGF1a in the sample or standard competes with a fixed amount of Universal 6-keto-PGF1a on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 6-keto-PGF1a. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 6-keto-PGF1a in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Citation 02

https://www.sciencedirect.com/science/article/pii/S075333222300118X

Citation 03

https://cmjournal.biomedcentral.com/articles/10.1186/s13020-021-00424-3

Citation 04

https://pubs.acs.org/doi/abs/10.1021/acsomega.0c05227

Citation 05

https://www.hindawi.com/journals/ecam/2021/9958808/

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of 6-keto-PGF1a concentrations in serum, plasma and other biological fluids.