Cr (Creatinine) ELISA Kit

Specifications

Catalog number

E-EL-0058-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Cr

Cross Reactivity

This kit recognizes Universal Cr in samples.No significant cross-reactivity or interference between Universal Cr and analogues was observed

Target

2-Amino-1-methyl-5H-imidazol-4-one;2-Amino-1-methylimidazol-4-ol

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal Cr. During the reaction, Universal Cr in the sample or standard competes with a fixed amount of Universal Cr on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal Cr. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal Cr in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.25-80 μg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.75 μg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332222013361

Citation 02

https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1751-7915.14200

Citation 03

https://www.mdpi.com/1436372

Citation 04

https://link.springer.com/article/10.1007/s12079-022-00685-z

Citation 05

https://www.mdpi.com/2218-1989/12/9/775

Citation 06

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202101030R

Citation 07

https://www.sciencedirect.com/science/article/pii/S0300483X22000270

Citation 08

https://pubmed.ncbi.nlm.nih.gov/33015817/

Citation 09

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0259132

Citation 10

https://www.sciencedirect.com/science/article/pii/S0211699521000576

Citation 11

https://link.springer.com/article/10.1007/s11255-020-02485-z

Citation 12

https://www.spandidos-publications.com/mmr/20/4/3337

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Cr concentrations in serum, plasma and other biological fluids.