ALD (Aldosterone) ELISA Kit

Specifications

Catalog number

E-EL-0070-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ALD

Cross Reactivity

This kit recognizes Universal ALD in samples.No significant cross-reactivity or interference between Universal ALD and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ALD. During the reaction, Universal ALD in the sample or standard competes with a fixed amount of Universal ALD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ALD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ALD in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ahajournals.org/doi/abs/10.1161/HYPERTENSIONAHA.119.12941

Citation 02

https://www.mdpi.com/article/10.3390/ijms232315400

Citation 03

https://www.mdpi.com/1847874

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332221003553

Citation 05

https://www.mdpi.com/2076-3921/11/5/1003

Citation 06

https://www.sciencedirect.com/science/article/pii/S2213231717302719

Citation 07

https://www.ahajournals.org/doi/abs/10.1161/JAHA.120.019961

Citation 08

https://www.hindawi.com/journals/omcl/2020/2978340/

Citation 09

https://www.sciencedirect.com/science/article/pii/S0753332220306764

Citation 10

https://www.frontiersin.org/articles/10.3389/fcimb.2017.00489/full

Citation 11

https://cdnsciencepub.com/doi/abs/10.1139/cjpp-2020-0278

Citation 12

https://www.tandfonline.com/doi/abs/10.1080/15376516.2018.1542474

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of ALD concentrations in serum, plasma and other biological fluids.