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Product details

ACH (Acetylcholine) ELISA Kit

Catalog number E-EL-0081-01

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Price for E-EL-0081-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-0081-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
ACH
Cross Reactivity
This kit recognizes Universal ACH in samples.No significant cross-reactivity or interference between Universal ACH and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Neuroscience; Signal transduction; Immunology
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ACH. During the reaction, Universal ACH in the sample or standard competes with a fixed amount of Universal ACH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ACH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ACH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202100808
Citation 02
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/
Citation 03
https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03896f
Citation 04
https://www.mdpi.com/1172448
Citation 05
https://www.sciencedirect.com/science/article/pii/S1319562X21005830
Citation 06
https://www.sciencedirect.com/science/article/pii/S0045653518321131
Citation 07
https://www.mdpi.com/1473434
Citation 08
https://www.mdpi.com/2075-1729/12/9/1406
Citation 09
https://www.mdpi.com/1261728
Citation 10
https://www.liebertpub.com/doi/abs/10.1089/jmf.2018.0197
Citation 11
https://www.sciencedirect.com/science/article/pii/S0304394019302137
Citation 12
https://www.hindawi.com/journals/ecam/2021/8884243/
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of ACH concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for ACH (Acetylcholine) ELISA Kit?

The catalog number for ACH (Acetylcholine) ELISA Kit is E-EL-0081.

What sizes are available for ACH (Acetylcholine) ELISA Kit?

ACH (Acetylcholine) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does ACH (Acetylcholine) ELISA Kit come in?

ACH (Acetylcholine) ELISA Kit is available in 3 different pack sizes.

Is ACH (Acetylcholine) ELISA Kit in stock?

ACH (Acetylcholine) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures ACH (Acetylcholine) ELISA Kit?

ACH (Acetylcholine) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for ACH (Acetylcholine) ELISA Kit?

Estimated delivery time for ACH (Acetylcholine) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for ACH (Acetylcholine) ELISA Kit?

The safety data sheet for ACH (Acetylcholine) ELISA Kit is available here: https://789.bio/eb/48a94K.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

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