CGMP (Cyclic GMP) ELISA Kit

Specifications

Catalog number

E-EL-0083-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

CGMP

Cross Reactivity

This kit recognizes Universal cGMP in samples.No significant cross-reactivity or interference between Universal cGMP and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal cGMP. During the reaction, Universal cGMP in the sample or standard competes with a fixed amount of Universal cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal cGMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal cGMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50 pmol/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 pmol/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353143/

Citation 02

https://www.sciencedirect.com/science/article/pii/S0147651322009198

Citation 03

https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000872&rev=1

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332222001536

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411812/

Citation 06

https://www.frontiersin.org/articles/10.3389/fendo.2021.636784/full

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.13807

Citation 08

https://journals.physiology.org/doi/abs/10.1152/ajpendo.00093.2021

Citation 09

https://www.sciencedirect.com/science/article/pii/S0378874121010552

Citation 10

https://www.sciencedirect.com/science/article/pii/S0378874121008746

Citation 11

https://pubs.acs.org/doi/abs/10.1021/acsomega.2c03888

Citation 12

https://www.sciencedirect.com/science/article/pii/S0166432822002777

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of cGMP concentrations in serum, plasma and other biological fluids.