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Product details

4-HNE (4-Hydroxynonenal) ELISA Kit

Catalog number E-EL-0128-01

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Price for E-EL-0128-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-0128-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
4-HNE
Cross Reactivity
This kit recognizes Universal 4-HNE in samples.No significant cross-reactivity or interference between Universal 4-HNE and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Neuroscience; Cell Biology; Metabolism
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 4-HNE. During the reaction, Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.63-40 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.38 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S1385894722044709
Citation 02
https://www.sciencedirect.com/science/article/pii/S014296122200360X
Citation 03
https://www.sciencedirect.com/science/article/pii/S2213231721002895
Citation 04
https://www.sciencedirect.com/science/article/pii/S0144861722002302
Citation 05
https://www.sciencedirect.com/science/article/pii/S0891584922005822
Citation 06
https://www.mdpi.com/2163138
Citation 07
https://www.hindawi.com/journals/omcl/2023/8258354/
Citation 08
https://www.sciencedirect.com/science/article/pii/S089158492200020X
Citation 09
https://www.sciencedirect.com/science/article/pii/S0753332222010204
Citation 10
https://www.sciencedirect.com/science/article/pii/S0048969720329429
Citation 11
https://www.mdpi.com/1576784
Citation 12
https://www.mdpi.com/1033142
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of 4-HNE concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for 4-HNE (4-Hydroxynonenal) ELISA Kit?

The catalog number for 4-HNE (4-Hydroxynonenal) ELISA Kit is E-EL-0128.

What sizes are available for 4-HNE (4-Hydroxynonenal) ELISA Kit?

4-HNE (4-Hydroxynonenal) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does 4-HNE (4-Hydroxynonenal) ELISA Kit come in?

4-HNE (4-Hydroxynonenal) ELISA Kit is available in 3 different pack sizes.

Is 4-HNE (4-Hydroxynonenal) ELISA Kit in stock?

4-HNE (4-Hydroxynonenal) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures 4-HNE (4-Hydroxynonenal) ELISA Kit?

4-HNE (4-Hydroxynonenal) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for 4-HNE (4-Hydroxynonenal) ELISA Kit?

Estimated delivery time for 4-HNE (4-Hydroxynonenal) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for 4-HNE (4-Hydroxynonenal) ELISA Kit?

The safety data sheet for 4-HNE (4-Hydroxynonenal) ELISA Kit is available here: https://789.bio/eb/bjTWX1.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

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