- Catalog number
- E-EL-0128-01
- Size / count
- 24 Tests
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Abbreviation
- 4-HNE
- Cross Reactivity
- This kit recognizes Universal 4-HNE in samples.No significant cross-reactivity or interference between Universal 4-HNE and analogues was observed
- Type
- Competitive-ELISA
- Applications
- ELISA
- Field of Research
- Neuroscience; Cell Biology; Metabolism
- Assay Principle
- This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 4-HNE. During the reaction, Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
- Assay Performance Time
- 2 h 30 min
- Sample Type
- Serum, plasma and other biological fluids
- Detection Range
- 0.63-40 ng/mL
- Precision
- Both intra-CV and inter-CV are < 10%.
- Recovery
- 80%-120%
- Sensitivity
- 0.38 ng/mL
- Shipping Conditions
- Ice bag
- Storage Conditions
- 2-8°C,12 months
- Citation 01
- https://www.sciencedirect.com/science/article/pii/S1385894722044709
- Citation 02
- https://www.sciencedirect.com/science/article/pii/S014296122200360X
- Citation 03
- https://www.sciencedirect.com/science/article/pii/S2213231721002895
- Citation 04
- https://www.sciencedirect.com/science/article/pii/S0144861722002302
- Citation 05
- https://www.sciencedirect.com/science/article/pii/S0891584922005822
- Citation 06
- https://www.mdpi.com/2163138
- Citation 07
- https://www.hindawi.com/journals/omcl/2023/8258354/
- Citation 08
- https://www.sciencedirect.com/science/article/pii/S089158492200020X
- Citation 09
- https://www.sciencedirect.com/science/article/pii/S0753332222010204
- Citation 10
- https://www.sciencedirect.com/science/article/pii/S0048969720329429
- Citation 11
- https://www.mdpi.com/1576784
- Citation 12
- https://www.mdpi.com/1033142
- Detection Instrument
- Microplate reader
- Species
- Universal
- Recommended Usage
- This ELISA kit applies to the in vitro quantitative determination of 4-HNE concentrations in serum, plasma and other biological fluids.