TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit

Specifications

Catalog number

E-EL-0162-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

TGF-β1

UniProt

H:P01137; M:P04202; R:P17246

Cross Reactivity

This kit recognizes Universal TGF-β1 in samples.No significant cross-reactivity or interference between Universal TGF-β1 and analogues was observed

Target

Cartilage-inducing factor; CED; Differentiation inhibiting factor; DPD1; LAP; Latency-associated peptide; Prepro transforming growth factor beta 1; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor; beta 1; Transforming Growth Factor-ß1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Universal TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Universal TGF-β1. You can calculate the concentration of Universal TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1385894722059460

Citation 02

https://www.sciencedirect.com/science/article/pii/S0142961222003258

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/all.15163

Citation 04

https://www.sciencedirect.com/science/article/pii/S1385894722011925

Citation 05

https://link.springer.com/article/10.1007/s42765-022-00224-w

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201661

Citation 07

https://pubs.acs.org/doi/abs/10.1021/acsami.2c13976

Citation 08

https://pubs.acs.org/doi/abs/10.1021/acsami.2c14448

Citation 09

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01461-2

Citation 10

https://www.sciencedirect.com/science/article/pii/S0168365921002996

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of TGF-β1 concentrations in Serum, plasma and other biological fluids.