F-TESTO (Free Testosterone) ELISA Kit

Specifications

Catalog number

E-EL-0165-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

F-TESTO

Cross Reactivity

This kit recognizes Universal F-TESTO in samples.No significant cross-reactivity or interference between Universal F-TESTO and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Epigenetics and Nuclear Signal; Signal transduction; Developmental biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal F-TESTO. During the reaction, Universal F-TESTO in the sample or standard competes with a fixed amount of Universal F-TESTO on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal F-TESTO. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal F-TESTO in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0147651321004371

Citation 02

https://www.sciencedirect.com/science/article/pii/S1319562X21003909

Citation 03

https://www.sciencedirect.com/science/article/pii/S0890623815300113

Citation 04

https://www.sciencedirect.com/science/article/pii/S0887233319305478

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025583/

Citation 06

https://link.springer.com/article/10.1007/s12011-017-1023-1

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.12490

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.12439

Citation 09

https://www.sciencedirect.com/science/article/pii/S1642431X22000328

Citation 10

http://www.publish.csiro.au/RD/RD18505

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1111/and.13137

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12930

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of F-TESTO concentrations in serum, plasma.