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Product details

Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit

Catalog number E-EL-H0035-01

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Price for E-EL-H0035-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H0035-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
SCD40L
UniProt
P29965
Cross Reactivity
This kit recognizes Human sCD40L in samples. No significant cross-reactivity or interference between Human sCD40L and analogues was observed
Target
TNFSF5; CD40LG; CD154; HIGM1; IGM; IMD3; T-BAM; TRAP; Gp39
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sCD40L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sCD40L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sCD40L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sCD40L. You can calculate the concentration of Human sCD40L in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.5-4000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
37.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep4.1440
Citation 02
https://www.sciencedirect.com/science/article/pii/S1590865822007290
Citation 03
https://www.hindawi.com/journals/ecam/2022/7941039/
Citation 04
https://www.mdpi.com/497180
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human sCD40L concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit?

The catalog number for Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is E-EL-H0035.

What sizes are available for Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit?

Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit come in?

Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is available in 3 different pack sizes.

Is Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit in stock?

Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit?

Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit?

Estimated delivery time for Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit?

The safety data sheet for Human sCD40L (Soluble Cluster of Differentiation 40 Ligand) ELISA Kit is available here: https://789.bio/eb/i1erXL.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

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