Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit

Specifications

Catalog number

E-EL-H0044-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

CX3CL1

UniProt

P78423

Cross Reactivity

This kit recognizes Human CX3CL1 in samples. No significant cross-reactivity or interference between Human CX3CL1 and analogues was observed

Target

Fractalkine; FKN; ABCD-3; C3Xkine; CXC3; CXC3C; NTN; NTT; SCYD1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CX3CL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CX3CL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CX3CL1. You can calculate the concentration of Human CX3CL1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.atsjournals.org/doi/abs/10.1165/rcmb.2018-0075OC

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.24579

Citation 03

https://link.springer.com/content/pdf/10.1007/s00408-018-0176-9.pdf

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human CX3CL1 concentrations in serum, plasma and other biological fluids.