Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit

Specifications

Catalog number

E-EL-H0050-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IP-10; CXCL10

UniProt

P02778

Cross Reactivity

This kit recognizes Human IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Human IP-10/CXCL10 and analogues was observed

Target

C7; IFI10; INP10; SCYB10; crg-2; gIP-10; mob-1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IP-10/CXCL10. You can calculate the concentration of Human IP-10/CXCL10 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8511489/

Citation 03

https://www.mdpi.com/1422-0067/23/19/11817

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.17236

Citation 05

https://jlb.onlinelibrary.wiley.com/doi/abs/10.1002/JLB.5HI1020-704R

Citation 06

https://www.nature.com/articles/s41598-021-01217-2

Citation 07

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270552

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.