Human Cyt-C (Cytochrome C) ELISA Kit

Specifications

Catalog number

E-EL-H0056-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Cyt-C

UniProt

P99999

Cross Reactivity

This kit recognizes Human Cyt-C in samples. No significant cross-reactivity or interference between Human Cyt-C and analogues was observed

Target

CytC; CYCS; CYC; HCS; THC4

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cyt-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cyt-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cyt-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Cyt-C. You can calculate the concentration of Human Cyt-C in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332221004893

Citation 02

https://www.sciencedirect.com/science/article/pii/S0891584916000083

Citation 03

https://www.mdpi.com/2075-1729/12/3/432

Citation 04

https://www.sciencedirect.com/science/article/pii/S0145305X18305615

Citation 05

https://academic.oup.com/jbcr/article-abstract/40/3/355/5423598

Citation 06

https://www.sciencedirect.com/science/article/pii/S2352340916300270

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc6129372/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Cyt-C concentrations in serum, plasma and other biological fluids.