Human FGF21 (Fibroblast Growth Factor 21) ELISA Kit

Specifications

Catalog number

E-EL-H0074-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

FGF21

UniProt

Q9NSA1

Cross Reactivity

This kit recognizes Human FGF21 in samples. No significant cross-reactivity or interference between Human FGF21 and analogues was observed

Target

FGF-21

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF21. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF21. You can calculate the concentration of Human FGF21 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2076-3921/9/8/759

Citation 02

https://europepmc.org/article/pmc/pmc8493335?javascript_support=no

Citation 03

https://www.mdpi.com/1422-0067/23/10/5490

Citation 04

https://www.mdpi.com/1251082

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/bcpt.13775

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1111/ggi.14263

Citation 07

https://www.sciencedirect.com/science/article/pii/S105687272100060X

Citation 08

https://www.degruyter.com/document/doi/10.1515/biol-2022-0095/html

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human FGF21 concentrations in serum, plasma and other biological fluids.