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Product details

Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit

Catalog number E-EL-H0079-01

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Price for E-EL-H0079-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H0079-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
G-CSF
UniProt
P09919
Cross Reactivity
This kit recognizes Human G-CSF in samples. No significant cross-reactivity or interference between Human G-CSF and analogues was observed
Target
CSF3; C17orf33; CSF3OS; Pluripoietin; Filgrastim; Lenograstim
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human G-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human G-CSF. You can calculate the concentration of Human G-CSF in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
39.06-2500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
23.44 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/
Citation 02
https://www.frontiersin.org/articles/10.3389/fimmu.2020.574270/full
Citation 03
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8845158/
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human G-CSF concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit?

The catalog number for Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit is E-EL-H0079.

What sizes are available for Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit?

Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit come in?

Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit is available in 3 different pack sizes.

Is Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit in stock?

Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit?

Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit?

Estimated delivery time for Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit?

The safety data sheet for Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit is available here: https://789.bio/eb/b18Ca5.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

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