Human IFN-γ (Interferon Gamma) ELISA Kit

Specifications

Catalog number

E-EL-H0108-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IFN-γ

UniProt

P01579

Cross Reactivity

This kit recognizes Human IFN-γ in samples. No significant cross-reactivity or interference between Human IFN-γ and analogues was observed

Target

IFNG; IFG; IFI; Type II Interferon

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-γ. You can calculate the concentration of Human IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41392-021-00703-3

Citation 02

https://jeccr.biomedcentral.com/articles/10.1186/s13046-020-01752-2

Citation 03

https://www.sciencedirect.com/science/article/pii/S2211383521002653

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/2162402X.2022.2079182

Citation 05

https://pubs.rsc.org/en/content/articlelanding/2021/nr/d1nr00132a

Citation 06

https://www.mdpi.com/2072-6643/14/24/5240

Citation 07

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d2fo00477a

Citation 08

https://www.hindawi.com/journals/omcl/2022/5249367/

Citation 09

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03896f

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29713

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517782/

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1002/clt2.12114

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IFN-γ concentrations in serum, plasma and other biological fluids.