Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Q: What is the catalog number for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

The catalog number for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is E-EL-H0109.

Q: What sizes are available for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

Q: How many pack sizes does Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit come in?

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available in 3 different pack sizes.

Q: Is Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit in stock?

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Q: Who manufactures Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is manufactured by Elabscience.

Q: What is the estimated delivery time for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

Estimated delivery time for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is approximately 15 business day(s), depending on your location.

Q: Where can I find the safety data sheet (SDS/MSDS) for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

The safety data sheet for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available here: https://789.bio/eb/uj1O0G.

Q: Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

Q: What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

Q: What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Catalog number E-EL-H0109-01

Choose a variant (3 options)

Selecting a variant updates its price, size and availability.

Price for E-EL-H0109-01

Size / count 24 Tests

Specifications

Catalog number: E-EL-H0109-01
Size / count: 24 Tests
Dry ice: No
Hazardous: No
Discontinued: No
Abbreviation: TNF-α
UniProt: P01375
Cross Reactivity: This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed
Target: DIF; TNF-alpha; TNFA; TNFSF2
Type: Sandwich-ELISA
Applications: ELISA
Field of Research: Cancer; Cardiovascular; Metabolism; Immunology; Signal transduction
Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time: 3 h 30 min
Sample Type: Serum, plasma and other biological fluids
Detection Range: 7.81-500 pg/mL
Precision: Both intra-CV and inter-CV are < 10%.
Recovery: 80%-120%
Sensitivity: 4.69 pg/mL
Shipping Conditions: Ice bag
Storage Conditions: 2-8°C,12 months
Citation 01: https://www.sciencedirect.com/science/article/pii/S009286742031000X
Citation 02: https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.31056
Citation 03: https://pubs.acs.org/doi/abs/10.1021/acsnano.6b06658
Citation 04: https://jeccr.biomedcentral.com/articles/10.1186/s13046-020-01752-2
Citation 05: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/
Citation 06: https://academic.oup.com/ecco-jcc/advance-article-abstract/doi/10.1093/ecco-jcc/jjac171/6794137
Citation 07: https://link.springer.com/article/10.1186/s12951-022-01288-x
Citation 08: https://www.sciencedirect.com/science/article/pii/S0269749122014312
Citation 09: https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02535-6
Citation 10: https://pubs.acs.org/doi/abs/10.1021/acsami.9b04827
Citation 11: https://www.nature.com/articles/s41419-021-03777-7
Citation 12: https://www.sciencedirect.com/science/article/pii/S0304383522000040
Detection Instrument: Microplate reader
Species: Human
Recommended Usage: This ELISA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

The catalog number for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is E-EL-H0109.

What sizes are available for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit come in?

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available in 3 different pack sizes.

Is Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit in stock?

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

Estimated delivery time for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

The safety data sheet for Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available here: https://789.bio/eb/uj1O0G.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.