• Certified Lab Equipment & Reagents
  • Fast Dispatch on In-Stock Items
  • Expert Scientific Support

Product details

Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit

Catalog number E-EL-H0111-01

Selecting a variant updates its price, size and availability.

Price for E-EL-H0111-01

Sign In for Pricing

Size / count 24 Tests

Specifications

Catalog number
E-EL-H0111-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
VEGF-A
UniProt
P15692
Cross Reactivity
This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed
Target
VEGF-A; VEGFA; MVCD1; VPF
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Metabolism; Developmental biology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-A. You can calculate the concentration of Human VEGF-A in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0142961222003957
Citation 02
https://www.sciencedirect.com/science/article/pii/S1385894722011925
Citation 03
https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01461-2
Citation 04
https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13316
Citation 05
https://clincancerres.aacrjournals.org/content/25/6/1957.abstract
Citation 06
https://www.nature.com/articles/s41419-022-04734-8
Citation 07
https://www.sciencedirect.com/science/article/pii/S0141813022022462
Citation 08
https://www.sciencedirect.com/science/article/pii/S0304383520304444
Citation 09
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-02739-w
Citation 10
https://www.mdpi.com/2072-6694/14/14/3365
Citation 11
https://www.sciencedirect.com/science/article/pii/S1742706119303277
Citation 12
https://www.sciencedirect.com/science/article/pii/S0378517322011206
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit?

The catalog number for Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is E-EL-H0111.

What sizes are available for Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit?

Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit come in?

Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is available in 3 different pack sizes.

Is Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit in stock?

Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit?

Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit?

Estimated delivery time for Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit?

The safety data sheet for Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is available here: https://789.bio/eb/yTC0uP.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

You Might Also Like
CD31 / PECAM-1 (158-2B3), CF488A conjugate, 0.1mg/mL
CD31 / PECAM-1 (158-2B3), CF488A conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC880352-100
Cytokeratin 8/18 (C-51), CF405S conjugate, 0.1mg/mL
Cytokeratin 8/18 (C-51), CF405S conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC040034-100
MUC2 (CCP58), CF647 conjugate, 0.1mg/mL
MUC2 (CCP58), CF647 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC470032-500
E-Cadherin / CD324 (Intercellular Junction Marker) (4A2), CF640R conjugate, 0.1mg/mL
E-Cadherin / CD324 (Intercellular Junction Marker) (4A2), CF640R conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC401429-100
CD46 (122.2), CF740 conjugate, 0.1mg/mL
CD46 (122.2), CF740 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC740175-500
E-Cadherin / CD324 (Intercellular Junction Marker) (4A2), CF647 conjugate, 0.1mg/mL
E-Cadherin / CD324 (Intercellular Junction Marker) (4A2), CF647 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC471429-100
Fibronectin (TV-1), CF568 conjugate, 0.1mg/mL
Fibronectin (TV-1), CF568 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC680029-500
Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/1325), CF647 conjugate, 0.1mg/mL
Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/1325), CF647 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC471325-100