Human Bcl-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit

Specifications

Catalog number

E-EL-H0114-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Bcl-2

UniProt

P10415

Cross Reactivity

This kit recognizes Human Bcl-2 in samples. No significant cross-reactivity or interference between Human Bcl-2 and analogues was observed

Target

Bcl2; PPP1R50

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Bcl-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Bcl-2. You can calculate the concentration of Human Bcl-2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.1c02037

Citation 02

https://www.sciencedirect.com/science/article/pii/S2352396419303275

Citation 03

https://www.mdpi.com/1172448

Citation 04

https://www.sciencedirect.com/science/article/pii/S0024320522003095

Citation 05

https://www.tandfonline.com/doi/abs/10.1080/14737159.2022.2016394

Citation 06

https://www.frontiersin.org/articles/10.3389/fcimb.2021.666900/full

Citation 07

https://www.mdpi.com/1420-3049/27/22/7993

Citation 08

https://www.mdpi.com/1420-3049/27/17/5389

Citation 09

https://www.sciencedirect.com/science/article/pii/S002228602201910X

Citation 10

https://pubs.acs.org/doi/abs/10.1021/acsomega.2c00028

Citation 11

https://link.springer.com/article/10.1007/s10072-021-05048-x

Citation 12

https://www.futuremedicine.com/doi/abs/10.2217/bmm-2021-0226

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.