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Product details

Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit

Catalog number E-EL-H0133-01

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Price for E-EL-H0133-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H0133-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
MMP-12
UniProt
P39900
Cross Reactivity
This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed
Target
MMP12; HME; ME; MME; Macrophage Metalloelastase; Macrophage Elastase
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-12. You can calculate the concentration of Human MMP-12 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.frontiersin.org/articles/10.3389/fimmu.2020.01902/full
Citation 02
https://www.thieme-connect.com/products/ejournals/html/10.1055/s-0043-122223
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human MMP-12 concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit?

The catalog number for Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit is E-EL-H0133.

What sizes are available for Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit?

Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit come in?

Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit is available in 3 different pack sizes.

Is Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit in stock?

Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit?

Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit?

Estimated delivery time for Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit?

The safety data sheet for Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit is available here: https://789.bio/eb/rvzvT8.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

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