Human AGT (Angiotensinogen) ELISA Kit

Specifications

Catalog number

E-EL-H0300-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

7732-18-5

Abbreviation

AGT

Synonyms

ANHU; SERPINA8;

UniProt

P01019

Species Reactivity

Human

Cross Reactivity

This kit recognizes Human AGT in samples. No significant cross-reactivity or interference between Human AGT and analogues was observed

Target

Human

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Detection Method

Colormetric

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AGT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AGT. You can calculate the concentration of Human AGT in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,12 months

Applications Notes

This ELISA kit applies to the in vitro quantitative determination of Human AGT concentrations in serum, plasma and other biological fluids.

Citation 01

https://journals.lww.com/md-journal/Fulltext/2022/02180/Effect_of_intrarenal_renin_angiotensin_aldosterone.27.aspx