Human APLN (Apelin) ELISA Kit

Specifications

Catalog number

E-EL-H0456-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

APLN

UniProt

Q9ULZ1

Cross Reactivity

This kit recognizes Human APLN in samples.No significant cross-reactivity or interference between Human APLN and analogues was observed

Target

APEL; XNPEP2

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human APLN. During the reaction, Human APLN in the sample or standard competes with a fixed amount of Human APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253381/

Citation 02

https://www.mdpi.com/2227-9059/10/7/1751

Citation 03

https://www.mdpi.com/1966912

Citation 04

https://www.mdpi.com/1446118

Citation 05

https://www.sciencedirect.com/science/article/pii/S0168822719302050

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1111/1744-9987.12412

Citation 07

https://journals.lww.com/md-journal/Fulltext/2021/03260/Association_of_serum_vaspin,_apelin,_and_visfatin.74.aspx

Citation 08

https://link.springer.com/article/10.1007/s12262-020-02440-3

Citation 09

https://www.sciencedirect.com/science/article/pii/S2452014421002909

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human APLN concentrations in serum, plasma and other biological fluids.