Human Aβ1-40 (Amyloid Beta 1-40) ELISA Kit

Specifications

Catalog number

E-EL-H0542-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Aβ1-40

UniProt

P05067

Cross Reactivity

This kit recognizes Human Aβ1-40 in samples. No significant cross-reactivity or interference between Human Aβ1-40 and analogues was observed

Target

Abeta 40; amyloid beta 40; Beta-amyloid protein 40; Aβ (1-40) ; Aβ40; Amyloid Beta 40

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Aβ1-40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Aβ1-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Aβ1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Aβ1-40. You can calculate the concentration of Human Aβ1-40 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1934590919305739

Citation 02

https://www.sciencedirect.com/science/article/pii/S2452199X21004321

Citation 03

https://www.sciencedirect.com/science/article/pii/S0168365923000834

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/15548627.2019.1596488

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/acel.13469

Citation 06

https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02521-y

Citation 07

https://www.sciencedirect.com/science/article/pii/S2211124718309045

Citation 08

https://www.frontiersin.org/articles/10.3389/fcell.2021.752753/full

Citation 09

https://www.sciencedirect.com/science/article/pii/S014181302201176X

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1111/tra.12874

Citation 11

https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b00377

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125555/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Aβ1-40 concentrations in serum, plasma and other biological fluids.