Human TP53 (Tumor Protein p53) ELISA Kit

Specifications

Catalog number

E-EL-H0910-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

TP53

UniProt

P04637

Cross Reactivity

This kit recognizes Human TP53 in samples.No significant cross-reactivity or interference between Human TP53 and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Epigenetics and Nuclear Signaling

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TP53. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TP53. You can calculate the concentration of Human TP53 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.1c02037

Citation 02

https://www.mdpi.com/1422-0067/23/9/5019

Citation 03

https://www.sciencedirect.com/science/article/pii/S0300908418302372

Citation 04

https://journals.sagepub.com/doi/abs/10.1177/0960327117737145

Citation 05

https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-020-1049-1

Citation 06

https://www.ajol.info/index.php/ahs/article/view/224630

Citation 07

https://www.sciencedirect.com/science/article/pii/S1658361221002365

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human TP53 concentrations in serum, plasma and other biological fluids.