Human β-CTx (Beta Crosslaps) ELISA Kit

Specifications

Catalog number

E-EL-H0960-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Β-CTx

UniProt

P02452

Cross Reactivity

This kit recognizes Human β-CTx in samples.No significant cross-reactivity or interference between Human β-CTx and analogues was observed

Target

B-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal transduction; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human β-CTx. During the reaction, Human β-CTx in the sample or standard competes with a fixed amount of Human β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

125-8000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101466/

Citation 02

https://www.sciencedirect.com/science/article/pii/S8756328218302461

Citation 03

https://europepmc.org/article/med/32967402?javascript_support=no

Citation 04

https://www.mdpi.com/2076-3417/12/16/8054

Citation 05

https://www.spandidos-publications.com/etm/18/2/1391

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human β-CTx concentrations in serum, plasma and other biological fluids.