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Product details

Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit

Catalog number E-EL-H0996-01

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Price for E-EL-H0996-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H0996-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
DEFβ2; DEFB2
UniProt
O15263
Cross Reactivity
This kit recognizes Human DEFβ2/DEFB2 in samples. No significant cross-reactivity or interference between Human DEFβ2/DEFB2 and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Microbiology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DEFβ2/DEFB2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFβ2/DEFB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFβ2/DEFB2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DEFβ2/DEFB2. You can calculate the concentration of Human DEFβ2/DEFB2 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.5-4000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
37.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.frontiersin.org/articles/10.3389/fcimb.2021.666900/full
Citation 02
https://www.mdpi.com/2142164
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human DEFβ2/DEFB2 concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit?

The catalog number for Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit is E-EL-H0996.

What sizes are available for Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit?

Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit come in?

Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit is available in 3 different pack sizes.

Is Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit in stock?

Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit?

Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit?

Estimated delivery time for Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit?

The safety data sheet for Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit is available here: https://789.bio/eb/yLW1eH.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

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